Towards a better recording of microtubule cytoskeletal spatial organization and dynamics in plant cells |
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Authors: | Weiwei Liu Chaofeng Wang Guangda Wang Yinping Ma Juan Tian Yanjun Yu Li Dong Zhaosheng Kong |
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Affiliation: | 1.State Key Laboratory of Plant Genomics, Institute of Microbiology, the Chinese Academy of Sciences, Beijing 100101, China;2.The University of Chinese Academy of Sciences, Beijing 100049, China ;These authors contributed equally to this work. ;Correspondence:;Email: Zhaosheng Kong (zskong@im.ac.cn) |
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Abstract: | Numerous fluorescent marker lines are currently available to visualize microtubule(MT)architecture and dynamics in living plant cells, such as markers expressing p35S::GFP-MBD or p35S::GFP-TUB6.However, these MT marker lines display obvious defects that affect plant growth or produce unstable fluorescent signals. Here, a series of new marker lines were developed, including the pTUB6::VisGreen-TUB6-expressing line in which TUB6 is under the control of its endogenous regulatory elements and e GFP is replaced with VisGreen, a brighter fluorescent protein. Moreover, two different markers were combined into one expression vector and developed two dual-marker lines.These marker lines produce bright, stable fluorescent signals in various tissues, and greatly shorten the screening process for generating dual-marker lines.These new marker lines provide a novel resource for MT research. |
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