Cloning, sequencing, and expression of cscA invertase from Escherichia coli B-62. |
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| Authors: | M Sahin-Tóth Z Lengyel H Tsunekawa |
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| Institution: | Department of Physiology, University of California Los Angeles 90095-1662, USA. miklos@hhmi.ucla.edu |
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| Abstract: | We have isolated a 2.5-kb DNA fragment from plasmid pST5R7 encoding a sucrose utilization system from Escherichia coli B-62 which confers a sucrose-fermenting phenotype to transformed E. coli K-12 strains. DNA-sequence determination revealed one full-length open reading frame 98% identical to cscA, the sucrose-hydrolase (invertase) gene of the csc regulon from E. coli EC3132. Functional characterization indicates that high-level expression and limited periplasmic release of invertase is responsible for the sucrose-fermenting capacity of transformed E. coli K-12 strains carrying cscA. |
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