Systematic approaches to using the FOX hunting system to identify useful rice genes |
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Authors: | Kondou Youichi Higuchi Mieko Takahashi Shinya Sakurai Tetsuya Ichikawa Takanari Kuroda Hirofumi Yoshizumi Takeshi Tsumoto Yuko Horii Yoko Kawashima Mika Hasegawa Yukako Kuriyama Tomoko Matsui Keiko Kusano Miyako Albinsky Doris Takahashi Hideki Nakamura Yukiko Suzuki Makoto Sakakibara Hitoshi Kojima Mikiko Akiyama Kenji Kurotani Atsushi Seki Motoaki Fujita Miki Enju Akiko Yokotani Naoki Saitou Tsutomu Ashidate Kozue Fujimoto Naka Ishikawa Yasuo Mori Yayoi Nanba Rie Takata Kazumasa Uno Kuniko Sugano Shoji Natsuki Jun Dubouzet Joseph Gogo Maeda Satoru Ohtake Miki Mori Masaki Oda Kenji |
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Affiliation: | RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045 Japan,; Research Institute for Biological Sciences Okayama, 7549-1 Yoshikawa, Kibi-chuo, Okayama 716-1241 Japan, and; Disease Resistance Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kan-nondai, Tsukuba, Ibaraki 305-8602, Japan |
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Abstract: | Ectopic gene expression, or the gain-of-function approach, has the advantage that once the function of a gene is known the gene can be transferred to many different plants by transformation. We previously reported a method, called FOX hunting, that involves ectopic expression of Arabidopsis full-length cDNAs in Arabidopsis to systematically generate gain-of-function mutants. This technology is most beneficial for generating a heterologous gene resource for analysis of useful plant gene functions. As an initial model we generated more than 23 000 independent Arabidopsis transgenic lines that expressed rice fl-cDNAs (Rice FOX Arabidopsis lines). The short generation time and rapid and efficient transformation frequency of Arabidopsis enabled the functions of the rice genes to be analyzed rapidly. We screened rice FOX Arabidopsis lines for alterations in morphology, photosynthesis, element accumulation, pigment accumulation, hormone profiles, secondary metabolites, pathogen resistance, salt tolerance, UV signaling, high light tolerance, and heat stress tolerance. Some of the mutant phenotypes displayed by rice FOX Arabidopsis lines resulted from the expression of rice genes that had no homologs in Arabidopsis . This result demonstrated that rice fl-cDNAs could be used to introduce new gene functions in Arabidopsis. Furthermore, these findings showed that rice gene function could be analyzed by employing Arabidopsis as a heterologous host. This technology provides a framework for the analysis of plant gene function in a heterologous host and of plant improvement by using heterologous gene resources. |
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Keywords: | rice Arabidopsis full-length cDNA FOX hunting system transgenic plants |
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