Type I callus as a bombardment target for generating fertile transgenic maize (Zea mays L.) |
| |
Authors: | Yuechun Wan Jack M Widholm Peggy G Lemaux |
| |
Institution: | (1) University of California-Berkeley/U.S. Department of Agriculture-Agricultural Research Service, Plant Gene Expression Center and Department of Plant Biology, 800 Buchanan Street, 94710 Albany, CA, USA;(2) Department of Agronomy, Plant and Animal Biotechnology Laboratory, University of Illinois, 61801 Urbana, IL, USA;(3) Present address: Monsanto Agricultural Company, 700 Chester-field Parkway North-GG4H, 63198 St. Louis, MO, USA |
| |
Abstract: | To develop a less genotype-dependent maize-transformation procedure, we used 10-month-old Type I callus as target tissue for microprojectile bombardment. Twelve transgenic callus lines were obtained from two of the three anther-culture-derived callus cultures representing different gentic backgrounds. Multiple fertile transgenic plants (T0) were regenerated from each transgenic callus line. Transgenic leaves treated with the herbicide Basta showed no symptoms, indicating that one of the two introduced genes, bar, was functionally expressing. Data from DNA hybridization analysis confirmed that the introduced genes (bar and uidA) were integrated into the plant genome and that all lines derived from independent transformation events. Transmission of the introduced genes and the functional expression of bar in T1 progeny was also confirmed. Germination of T1 immature embryos in the presence of bialaphos was used as a screen for functional expression of bar; however, leaf painting of T1 plants proved a more accurate predictor of bar expression in plants. This study suggests that maize Type I callus can be transformed efficiently through microprojectile bombardment and that fertile transgenic plants can be recovered. This system should facilitate the direct introduction of agronomically important genes in to commercial genotypes. |
| |
Keywords: | Transformation Transgene expression Transgene segregation Type I callus Zea (transformation) |
本文献已被 SpringerLink 等数据库收录! |
|