The subunit structure of chromatin fibres |
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Authors: | Juan A Subirana Sebastián Muñoz-Guerra Antonio B Martínez Luis Pérez-Grau Xavier Marcet Ignacio Fita |
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Institution: | (1) Unidad de Química Macromolecular del C.S.I.C., Escuela T.S. de Ingenieros Industriales, Diagonal, 999, Barcelona 28, Spain |
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Abstract: | Optimal conditions for studying the ultrastructure of chromatin fibers of histone-containing spermatozoa in thin sections have been determined. Better results for preservation in sperm of the sea cucumber Holothuria tubulosa, have been found than in different frog species studied. The fine structure of chromatin fibers after different treatments was studied by computer methods. A clear superbead structure was found under all conditions which preserve the chromatin fibres. These have a diameter of 30 nm, with superbeads about 33 nm long. In the best preserved cases an additional periodicity of 11 nm along the fibres was found. There is no clear relationship of this periodicity with an eventual solenoidal structure of the chromatin fibers. |
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