Efficacy of bacterial consortium-AIE2 for contemporaneous Cr(VI) and azo dye bioremediation in batch and continuous bioreactor systems, monitoring steady-state bacterial dynamics using qPCR assays |
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Authors: | Chirayu Desai Kunal Jain Bharat Patel Datta Madamwar |
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Institution: | (1) Microbiology Research Division, BRD School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, Gujarat, 388120, India;(2) School of Biomolecular and Physical Sciences, Griffith University, Brisbane, QLD, 4111, Australia |
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Abstract: | Bacterial consortium-AIE2 with a capability of contemporaneous Cr(VI) reduction and azo dye RV5 decolourization was developed
from industrial wastewaters by enrichment culture technique. The 16S rRNA gene based molecular analyses revealed that the
consortium bacterial community structure consisted of four bacterial strains namely, Alcaligenes sp. DMA, Bacillus sp. DMB, Stenotrophomonas sp. DMS and Enterococcus sp. DME. Cumulative mechanism of Cr(VI) reduction by the consortium was determined using in vitro Cr(VI) reduction assays.
Similarly, the complete degradation of Reactive Violet 5 (RV5) dye was confirmed by FTIR spectroscopic analysis. Consortium-AIE2
exhibited simultaneous bioremediation efficiencies of (97.8 ± 1.4) % and (74.1 ± 1.2) % in treatment of both 50 mg l−1 Cr(VI) and RV5 dye concentrations within 48 h of incubation at pH 7 and 37°C in batch systems. Continuous bioreactor systems
achieved simultaneous bioremediation efficiencies of (98.4 ± 1.5) % and (97.5 ± 1.4) % after the onset of steady-state at
50 mg l−1 input Cr(VI) and 25 mg l−1 input RV5 concentrations, respectively, at medium dilution rate (D) of 0.014 h−1. The 16S rRNA gene copy numbers in the continuous bioreactor as determined by real-time PCR assay indicated that Alcaligenes sp. DMA and Bacillus sp. DMB dominated consortium bacterial community during the active continuous bioremediation process. |
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