Purification and Characterization of a Cold-active Iron Superoxide Dismutase from a Psychrophilic Bacterium, Marinomonas sp. NJ522 |
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| Authors: | Zhou Zheng Ying-Hui Jiang Jin-Lai Miao Quan-Fu Wang Bo-Tao Zhang Guang-You Li |
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| Institution: | (1) College of life Sciences, Ocean University of China, 266003 Qingdao, P.R. China;(2) Key Laboratory of Marine Bio-active Substances, State Oceanic Administration, First Institute of Oceanorgarphy, 266061 Qingdao, P.R. China;(3) Huangdao Entry-Exit Inspection and Quarantine of P.R China, 266555 Qingdao, P.R. China |
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| Abstract: | Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified
SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 °C (assayed over
10 min). Activity at 0 °C was nearly 35% of the maximum activity.
Received 25 August 2005; Revisions requested 30 August 2005 and 26 September 2005; Revisions received 12 September 2005 and
25 October 2005; Accepted 1 November 2005 |
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| Keywords: | Antarctic cold-active Marinomonas psychrophilic bacteria superoxide dismutase |
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