Pulsed field gel electrophoresis: Theory,instruments and application |
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Authors: | E. S. Nassonova |
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Affiliation: | (1) Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia |
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Abstract: | Pulsed field gel electrophoresis (PFGE) is a technique for the fractionation of high-molecular-weight DNA ranging from 10 kb to 10 Mb by electrophoresis in agarose gel with an electric field that alternates (pulsates) in two directions. This technology plays a key role in modern genomics, as it allows manipulations with DNA of whole chromosomes or their large fragments. In this review, we discuss (1) the theory behind PFGE; (2) different instruments based on the principle of pulsed field, as well as their advantages and limitations; (3) factors affecting the DNA mobility in PFGE gel; and (4) practical applications of the technique. |
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Keywords: | PFGE DNA fractionation chromosomal DNA |
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