| Does a proton-pumping ATPase exist in the tonoplast? |
| |
| 摘 要： |
|
| 收稿时间： | 1986-08-01 |
Does a proton-pumping ATPase exist in the tonoplast? |
| |
| Alain Dupaix, Max Hill, Pierre Volfin,Bernard Arrio. Does a proton-pumping ATPase exist in the tonoplast?[J]. Biochimie, 1986, 68(12): 1293-1298. DOI: 10.1016/S0300-9084(86)80081-5 |
| |
| Authors: | Alain Dupaix Max Hill Pierre Volfin Bernard Arrio |
| |
| Affiliation: | 1. Key Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China;2. Analytical & Testing Research Center, Kunming University of Science and Technology, Kunming, 650500, China;3. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650205, China;4. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijing, 100700, China;1. College of Life Science and Engineering, Shenyang University, Shenyang, 110044, China;2. Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Harbin, 150081, China;3. Northern Rice Research Center of Bao Qing, Shuangyashan, 155600, China;4. Institute of Agricultural and Animal Husbandry of Hinggan League, Inner Mongolia Key Laboratory of Rice Breeding Innovation in Northern Cold Regions, Ulanhot, 137400, China |
| |
| Abstract: | In order to account for the accumulation of metabolites in plant vacuoles, the existence of a proton-pumping ATPase has been widely suggested in the literature. The demonstration of such a tonoplast-bound ATPase was merely based on the characterization of a nitrate-sensitive microsomal fraction. In some examples, this ATPase activity has been evidenced on vacuole preparations obtained under conditions which were criticized by Boller. The application of the reverse phase high-performance liquid chromatography method (RP-HPLC) to the simultaneous separation of adenine nucleotides, in the presence of tonoplast vesicles isolated from Catharanthus roseus, showed results not necessarily correlated with the ATPase hypothesis. Moreover, in light of the H+-quenching of quinacrine fluorescence observed during ATP hydrolysis by vacuoles or tonoplast vesicles, the existence of a proton-pumping ATPase may be questioned. |
| |
| Keywords: | Catharanthus roseus tonoplaste ATPase RP-HPLC |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
