Topography of substrate-attached glycoproteins from normal and virus-transformed cells

The topographical distribution of substrate-attached material (SAM) which may mediate adhesion of BALB/c 3T3 and SV40-transformed 3T3 cells to the culture substrate has been examined at both the macroscopic and microscopic levels. Autoradiographic detection of these ‘glycoproteins’ on the substrate subsequent to incorporation of ¹⁴C-glucosamine or ³⁵S-methionine and exposure to X-ray film indicated:

1. 1. Evenly distributed glucosamine-labeled SAM on the substrate of confluent cultures.

2. 2. A deficiency in the methionine content of SAM from transformed cells.

3. 3. Direct deposition of these materials onto the substrate at cell colony locations, and not randomly on the substrate subsequent to secretion into the medium.

Autoradiographic detection of these ‘glycoproteins’ subsequent to ³H-glucosamine (or ³H-leucine) incorporation and exposure to liquid emulsion indicated:

1. 1. Substrate-attached glycoproteins are present in focal pools on the underside of the cell.

2. 2. These focal pools are distributed comparably, in terms of number of foci per area of substrate, for confluent normal and transformed cells. The patterns of SAM deposited by freshly-attaching and spreading 3T3 cells has also been examined. The evidence suggests that cells are adherent to the substrate at localized areas which appear to be evenly distributed on the underside of normal or transformed cells.