Preliminary x-ray diffraction studies on an anti-viral protein. |
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Authors: | J D Irvin J D Robertus A F Monzingo |
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Affiliation: | Clayton Foundation Biochemical Institute Department of Chemistry The University of Texas Austin, Texas 78702 USA;Department of Chemistry Southwest Texas State University San Marcos, Texas 78666 USA |
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Abstract: | An anti-viral enzyme from , known to inhibit protein synthesis has been crystallized in a form useful for high resolution x-ray diffraction studies. Cracking of crystals due to the introduction of heavy metals can be reduced by cross linking with glutaraldehyde using a rapid fixation method. Several apparent isomorphous heavy metal derivatives of the crystal have been found. The molecular weight of the protein has been reevaluated as 31,000 daltons. |
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Keywords: | T.f. CaBP calcium-binding protein inorganic phosphate |
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