| Abstract: | The effect of two Rec(-) mutations (AB2463 and JC1553) on the ability of a cell to accept, maintain, and express the colicinogenic factors ColE(1), ColE(2), and ColV was examined in Escherichia coli. These mutations had no observable effect on the colicinogenic properties of the ColV factor, but prevented the spontaneous and induced production of colicins E(1) and E(2) which are determined by the ColE(1) and ColE(2) factors, respectively. The two Rec(-) mutations had no apparent effect on the ability of the cells to acquire, maintain, or transfer the ColE(1) and ColE(2) factors. These mutations did not affect the expression of immunity by any of the three Col factors. ColE(1) and ColE(2) were also shown to be indirectly induced by mating F(-) cells carrying these Col factors with ultraviolet-irradiated, non-colicinogenic, Hfr and F(+) cells. Indirect induction of colicin production occurred when either an irradiated F(+) Rec(+) or F(+) Rec(-) strain was employed as the donor strain. |
