Caprine arthritis-encephalitis virus (CAEV) detection in semen of endangered goat breeds by nested polymerase chain reaction |
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Authors: | J.C.M. Cruz A.M.G. Gouveia K.C. Souza G.F. Braz B.M. Teixeira M.B. Heinemann R.C. Leite J.K.P. Reis R.R. Pinheiro A. Andrioli |
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Affiliation: | aVeterinary Preventive Medicine Department, Escola de Veterinária, Universidade Federal de Minas Gerais, C. Postal 567, 30161-970, Belo Horizonte, MG, Brazil;bBrazilian Agricultural Research Corporation, Embrapa, Centro Nacional de Pesquisa de Caprinos e Ovinos, Cx. Postal 10, 62011-970, Sobral, CE, Brazil |
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Abstract: | Semen and peripheral blood mononuclear cells (PBMCs) samples of four naturally infected, four experimentally infected (endangered breeds) and four non-infected bucks (endangered breeds) were evaluated for the presence of CAEV proviral-DNA by nested polymerase chain reaction (n-PCR). Three out of the eight PBMC samples from infected bucks were positive for CAEV-DNA and four out of the eight semen samples were positive for CAEV proviral-DNA. This is the first report describing the presence of CAEV proviral-DNA in semen from seropositive Anglo-Nubian, Moxotó and Canindé bucks, providing useful information towards the design of efficient methods to prevent CAEV dissemination in the endangered goat livestock genetic resources in Brazil. |
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Keywords: | Caprine arthritis-encephalitis virus Semen Nested-PCR Goat |
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