An organic solvent-tolerant alkaline lipase from <Emphasis Type="Italic">Streptomyces</Emphasis> sp. CS268 and its application in biodiesel production |
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Authors: | Poonam Mander Seung Sik Cho Jaya Ram Simkhada Yun Hee Choi Da Jeong Park Jung Wan Ha Jin Cheol Yoo |
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Institution: | 1.Department of Pharmacy, College of Pharmacy,Chosun University,Gwangju,Korea |
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Abstract: | In an effort to identify a microbial lipase that can catalyze transesterification reactions used in biodiesel production,
an organic solvent-tolerant lipase was purified from Streptomyces sp. CS268. The molecular weight of the purified lipase was estimated to be 37.5 kDa by SDS-PAGE. The lipase showed highest
activity at a temperature of 30°C and pH 8.0 while it was stable in the pH range 4.0 ∼ 9.0 and at temperatures ≤ 50°C. It
showed the highest hydrolytic activity towards medium-length acyl chain p-nitrophenyl decanoate with K
m
and V
max
values of 0.59 mM and 319.5 mmol/mg/min, respectively. Also, the lipase showed non-position specificity for triolein hydrolysis.
The purified lipase catalyzed transesterification reaction of soybean oil with methanol, suggesting that it can be a potential
enzymatic catalyst for biodiesel production. |
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Keywords: | |
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