Resistance to Cucumber mosaic virus in Gladiolus plants transformed with either a defective replicase or coat protein subgroup II gene from Cucumber mosaic virus |
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Authors: | Kathryn Kamo Ramon Jordan Mary Ann Guaragna Hei-ti Hsu Peter Ueng |
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Institution: | (1) Floral and Nursery Plants Research Unit, USDA, US National Arboretum, 10300 Baltimore Avenue, Building 10A Room 122, Beltsville, MD 20705, USA;(2) Molecular Plant Pathology Laboratory, USDA, Beltsville, MD 20705, USA |
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Abstract: | Transgenic Gladiolus plants that contain either Cucumber mosaic virus (CMV) subgroup I coat protein, CMV subgroup II coat protein, CMV replicase, a combination of the CMV subgroups I and II coat
proteins, or a combination of the CMV subgroup II coat protein and replicase genes were developed. These plants were multiplied
in vitro and challenged with purified CMV isolated from Gladiolus using a hand-held gene gun. Three out of 19 independently transformed plants expressing the replicase gene under control
of the duplicated CaMV 35S promoter were found to be resistant to CMV subgroup I. Three out of 21 independently transformed
plants with the CMV subgroup II coat protein gene under control of the Arabidopsis UBQ3 promoter were resistant to CMV subgroup II. Eighteen independently transformed plants with either the CMV subgroup I coat
protein or a combination of CMV subgroups I and II coat proteins were challenged and found to be susceptible to both CMV subgroups
I or II. Virus resistant plants with the CMV replicase transgene expressed much lower RNA levels than resistant plants expressing
the CMV subgroup II coat protein. This work will facilitate the evaluation of virus resistance in transgenic Gladiolus plants to yield improved floral quality and productivity. |
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