Atrial natriuretic peptide (ANP): a study of ANP and its mRNA in cardiocytes,and of plasma ANP levels in non-obese diabetic mice |
| |
| Authors: | Hiroharu Mifune Syusaku Suzuki Junichi Honda Yuta Kobayashi Yasutaka Noda Yoshihiro Hayashi Koshi Mochizuki |
| |
| Institution: | (1) Institute of Animal Experiment, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830 Fukuoka, Japan;(2) Institute of Laboratory Animal Sciences, Faculty of Medicine, Kagoshima University, Kagoshima, Japan;(3) Department of Internal Medicine, Kurume University School of Medicine, Fukuoka, Japan;(4) Department of Pharmacology, Shimane Medical University, Shimane, Japan;(5) Laboratory of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Tokyo, Japan;(6) Laboratory of Biomedical Science, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan |
| |
| Abstract: | Summary Atrial natriuretic peptide (ANP) levels in cardiocytes and plasma were examined by using immunohistochemistry, electron microscopy, and radioimmunoassay in non-obese diabetic mice (NOD). Cardiocyte ANP mRNA expression was measured by the polymerase chain reaction method. ANP immunoreactivity in the auricular cardiocytes was more prominent in hyperglycemic mice (NOD-h) than in normoglycemic mice (NOD-n). Ultrastructural examination showed that auricular cardiocytes of the NOD-h group contained more cytoplasmic granules than cells of the NOD-n group. Ultrastructural morphometry indicated that the number of granules per auricular cardiocyte was significantly larger in the NOD-h group than in the NOD-n group. (P<0.01), whereas the granule diameter was significantly smaller in the NOD-h group (P<0.01). Radioimmunoassay showed that ANP levels in the NOD-h auricular cardiocytes were significantly higher than those in the NOD-n cardiocytes (P<0.01); the opposite was true in plasma. Cardiocyte ANP mRNA expression was lower in the NOD-h group than in the NOD-n group. |
| |
| Keywords: | Atrial natriuretic peptides mRNA Diabetes type I Immunohistochemistry Morphometry Mouse (NOD) |
| 本文献已被 SpringerLink 等数据库收录！ |
|
