Comparative Evaluation of SSR and ISSR Markers for Polymorphism in Landraces and Elite Varieties of Rice (Oryza sativa L) |
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Authors: | A. S. Hari Prasad C. N. Neeraja N. Sarla E. A. Siddiq |
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Affiliation: | 1. Directorate of Rice Research, Hyderabad, 500 030, India
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Abstract: | The study aims at comparing SSR and ISSR markers for their ability to identify polymorphism in parents comprising three landraces and two elite varieties of rice. Both the SSR (0.51) and ISSR (0.46) primers showed almost similar values for Poly morphic Information Content (PIC). Maximum PIC values were observed with trinucleotide ISSR primers (0.67) followed by dinucleotide ISSR primers. In addition to the mapped SSR markers, ISSR markers used in the present study produced more polymorphic markers suggesting their utility in the survey of polymorphism between the parental lines belonging to the same sub-species of rice. |
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