A direct interaction of axonin-1 with NgCAM-related cell adhesion molecule (NrCAM) results in guidance, but not growth of commissural axons |
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Authors: | Fitzli D Stoeckli E T Kunz S Siribour K Rader C Kunz B Kozlov S V Buchstaller A Lane R P Suter D M Dreyer W J Sonderegger P |
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Affiliation: | Institute of Biochemistry, University of Zurich, Switzerland. |
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Abstract: | An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth. |
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Keywords: | axon guidance growth cone neuronal cell adhesion molecules immunoglobulin superfamily signal transduction |
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