Decalcified egg shell membrane,a supporting substrate for electron microscopic cross-sections of monolayers of epithelial cell line MDCK |
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Authors: | Joseph Leighton Sunder Mansukhani Larry W Estes |
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Institution: | (1) Cancer Bioassay Research Laboratory, Department of Pathology, University of Pittsburgh, 15213 Pittsburgh, Pennsylvania |
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Abstract: | Summary Decalcified chicken egg shell membrane is a suitable physical substrate for the growth of monolayers of an epithelial line
of tissue culture cells, MDCK. The shell membranes with their adherent sheets of cells are easily prepared for cross-section
study of monolayer growth with electron microscopy.
This study was supported by Research Grant P-442 from the American Cancer Society. |
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