Localization of the initial steps in alkoxyphospholipid biosynthesis in glycosomes (microbodies) of Trypanosoma brucei |
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| Authors: | F R Opperdoes |
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| Affiliation: | 1. A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 117234, USSR;2. Institute of Cytology and Genetics, Siberian Branch of the USSR Academy of Sciences, Novosibirsk 630090, USSR |
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| Abstract: | Cell fractionation of Trypanosoma brucei cultured procyclic stages showed that the key enzyme of glycerol-ether lipid synthesis, dihydroxyacetone-phosphate acyltransferase (EC 2.3.1.42) was exclusively associated with the microbody fraction. These organelles contained in addition 1-acyl glycerol-3-phosphate: NADP+ oxidoreductase (EC 1.1.1.101) and acyl-CoA reductase and were capable of utilizing DHAP, but not G-3-P, as substrate for lysophosphatidic acid formation. It is concluded that in T. brucei the glycosomes are the exclusive site of the synthesis of precursors for glycerol-ether lipid synthesis and that they contain the entire pathway to form alkoxylipids from glycerol and acyl-CoA. |
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