Survival of electrical activity of deep frozen fetal mouse hearts after microwave thawing |
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Authors: | R V Rajotte D W Jirsch J B Dossetor E Diener W A Voss |
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Institution: | MRC Transplantation Group, and Surgical Medical Research Institute, University of Alberta, Edmonton, Canada |
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Abstract: | Hearts removed from 17–19 day fetal mice were frozen in liquid nitrogen and tested for electrical activity after rewarming. After exposure to various cryoprotective agents, hearts were cooled at 0.5–0.7 °C/min. to ?100 °C and then stored in liquid nitrogen for periods between 72 and 216 hr. Exposure to controlled microwaves at 2450 MHz or immersion in a water bath at 25 C was used in thawing. Electrical activity was studied for periods as long as 90 days after subcutaneous implantation into the ear of syngeneic adult mice. Overall, 59% of 54 frozen-thawed fetal hearts showed strong electrical activity after 30 days when the cryoprotective solution that had been used contained 10% () dimethylsulfoxide (DMSO) and 10% () fetal calf serum in Hepes buffer. This system consists of a multicellular structure that is nourished by diffusion; it is well suited for the evaluation of different cryoprotective agents and for various thawing techniques. |
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