| 人脑源性神经营养因子cDNA在COS7细胞中的表达及活性研究 |
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| 引用本文： | 陈谦,汪家政,范明.人脑源性神经营养因子cDNA在COS7细胞中的表达及活性研究[J].生物化学与生物物理学报,1997(4). |
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| 作者姓名： | 陈谦 汪家政 范明 |
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| 作者单位： | 军事医学科学院基础医学研究所 |
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| 摘 要： | 本文从质粒Ｍ１３ｍｐ１８－ｈＢＤＮＦ中酶切回收人脑源性神经营养因子（ｈＢＤＮＦ）全长基因，构建真核表达载体ｐＣＭＶ４－ｈＢＤＮＦ。利用脂质体的方法转染ＣＯＳ７细胞，对转染后的ＣＯＳ７细胞提取ＲＮＡ进行狭缝杂交分析和免疫细胞化学反应，分别从转录及翻译水平上检测ＢＤＮＦ基因在ＣＯＳ７细胞中的表达。实验还证实在ＣＯＳ７细胞中表达的ｈＢＤＮＦ蛋白可分泌至胞外并可促进中脑黑质细胞的发育和生长，具有良好的生物学活性。
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| 关 键 词： | 脑源性神经生长因子（BDNF） 基因表达 狭缝杂交分析 免疫细胞化学反应 中脑黑质细胞培养 |
An Experimental X Band Super Resolution Synthetic Array Imaging System |
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| Qiu Xiaohui,Zhu Zhaoda,Zhang Xinggan,Yu Weidong,Shen Qingdu.An Experimental X Band Super Resolution Synthetic Array Imaging System[J].Acta Biochimica et Biophysica Sinica,1997(4). |
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| Authors: | Qiu Xiaohui Zhu Zhaoda Zhang Xinggan Yu Weidong Shen Qingdu |
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| Institution: | Qiu Xiaohui Zhu Zhaoda Zhang Xinggan Yu Weidong Shen Qingdu Department of Electrical Engineering,NUAA Nanjing,210016 |
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| Abstract: | An experimental X band super resolution synthetic array imaging system in microwave anechoic chamber is introduced. The system can provide high range resolution by using wide band LFM waveform and time band exchange technique. High cross range resolution can be obtained in the system through moving the receiving antenna a linear slideway to create a large synthetic aperture. The imaging results of processing the real data collected by the system are given in this paper. It is shown that super resolution approach may provide resolution advantage of a factor more than 2 compared with the conventional method. |
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| Keywords: | radar signal processing synthetic aperture radar image formation resolution |
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