| 两个不同地区东方田鼠杂交子代RAPD标记分析 |
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| 引用本文： | 邵伟娟,谢建云,王胜昌,高诚.两个不同地区东方田鼠杂交子代RAPD标记分析[J].中国实验动物学报,2001,9(2):93-98. |
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| 作者姓名： | 邵伟娟 谢建云 王胜昌 高诚 |
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| 作者单位： | 上海市实验动物质量监督检验站,上海 200032 |
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| 基金项目： | 上海市科技发展基金(984419074) |
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| 摘 要： | 目的研究不同地区东方田鼠杂交后产下的子代的遗传特性.方法筛选4条10bp随机引物对宁夏和洞庭湖地区东方田鼠杂交子代的基因组进行随机扩增多态DNA(RAPD)分析,并对不同地区亲代以及子代相互之间的基因组DNA进行相似性分析.结果①所有东方田鼠均有相同的扩增片段出现;②两个不同地区的亲代分别有特异性片般;③亲代的DNA带型均能在子代中找到;④同一胎次东方田鼠之间基因共享度大约在72%～96%之间.结论RAPD分析能在一定程度上反映出东方田鼠种的特性以及亚种的特异性,而且同一胎次之间基因共享度较高.
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| 关 键 词： | 杂交 亲子关系 田鼠亚科 随机扩增多态DNA分析法 |
| 文章编号： | 1005-4847(2001)02-0093-06 |
| 修稿时间： | 2000年8月20日 |
Genomic RAPD Analysis on Hybrid Field Voles(Microtus Fortis) from Two Regions in China |
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| SHAO Weijuan,XIE Jianyun,WANG Shengchang,GAO Cheng.Genomic RAPD Analysis on Hybrid Field Voles(Microtus Fortis) from Two Regions in China[J].Acta Laboratorium Animalis Scientia Sinica,2001,9(2):93-98. |
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| Authors: | SHAO Weijuan XIE Jianyun WANG Shengchang GAO Cheng |
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| Abstract: | Objective\ To research genetic type of hybrid field voles from different regions.Methods\ We used 4 10bp random primers to amplify DNA of hybrid field voles from Ningxia and Dongtinghu in China and analyzed the homology of parents and their offsprings.Results\ There were several fragments of genomic DNA shared by all field voles but specific fragments were seen in the parental generation of the two different regions. 4 primers could be used as characteristic RAPD markers to distinguish the subspecies.Parental RAPD markers were found in the offsprings.The homology of field voles of same litter were among 70%-96%.Conclusion\ RAPD could be used to distinguish the species or subspecies of field voles. |
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| Keywords: | Hybridization Parent-child relations Subspecies of voles Genomic DNA RAPD analysis |
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