Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L.) Raf. |
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| Authors: | P F Mestre M J Asíns J A Pina E A Carbonell L Navarro |
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| Institution: | (1) Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, E-46113 Moncada (Valencia), Spain, ES;(2) Servicio de Sanidad y Certificación Vegetal, Apartado Oficial, E-46113 Moncada, Spain, ES |
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| Abstract: | Two segregating populations for citrus tristeza virus (CTV) resistance derived from Poncirus trifoliata var ‘Flying Dragon’ by self-pollination and pollination to Citrus medica L. var ethrog ‘Arizona’ were inoculated with a common CTV isolate. The presence of virus was checked by the Double Antibody
Sandwich Enzyme-Linked Assay and Direct Tissue Blot Inmunoassay at 3, 6, and 12 months after inoculation. Seven RAPDs were
found linked to the CTV resistance gene by bulked segregant analysis. The closest linked RAPDs were cloned to obtain linked
codominant RFLPs and to increase the precision of the genetic distance estimation. The CTV resistance gene seems to be located
between cW18 and cK16. Differences in genetic distances among progenies are large and can be explained by genome-wide reduction
in the recombination of progeny derived from male versus female gametes.
Received: 5 June 1996 / Accepted: 26 July 1996 |
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| Keywords: | CTV resistance Molecular markers Recombination Marker-assisted selection Fruit breeding |
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