Physiological importance of RNA and protein mobility in the cell nucleus |
| |
Authors: | Tom Misteli |
| |
Institution: | (1) National Cancer Institute, National Institutes of Health, 41 Library Drive, Bethesda, MD 20892, USA |
| |
Abstract: | Trafficking of proteins and RNAs is essential for cellular function and homeostasis. While it has long been appreciated that
proteins and RNAs move within cells, only recently has it become possible to visualize trafficking events in vivo. Analysis
of protein and RNA motion within the cell nucleus have been particularly intriguing as they have revealed an unanticipated
degree of dynamics within the organelle. These methods have revealed that the intranuclear trafficking occurs largely by energy-independent
mechanisms and is driven by diffusion. RNA molecules and non-DNA binding proteins undergo constrained diffusion, largely limited
by the spatial constraint imposed by chromatin, and chromatin binding proteins move by a stop-and-go mechanism where their
free diffusion is interrupted by random association with the chromatin fiber. The ability and mode of motion of proteins and
RNAs has implications for how they find nuclear targets on chromatin and in nuclear subcompartments and how macromolecular
complexes are assembled in vivo. Most importantly, the dynamic nature of proteins and RNAs is emerging as a means to control
physiological cellular responses and pathways. |
| |
Keywords: | Nuclear architecture Dynamics Diffusion RNA |
本文献已被 PubMed SpringerLink 等数据库收录! |
|