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| 粉红黏帚霉67-1菌株寄生核盘菌菌核的酶学动态研究 | |
| 引用本文： | 李晶晶,张拥华,陈巧云,李世东,谢响明.粉红黏帚霉67-1菌株寄生核盘菌菌核的酶学动态研究[J].菌物研究,2007,5(2):107-109. |
| 作者姓名： | 李晶晶 张拥华 陈巧云 李世东 谢响明 |
| 作者单位： | 1. 北京林业大学生物科学与技术学院,北京,100083;中国农业科学院植物保护研究所,北京,100081 2. 中国农业科学院植物保护研究所,北京,100081 3. 北京林业大学生物科学与技术学院,北京,100083 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 对粉红黏帚霉67-1菌株侵染核盘菌菌核过程的多种细胞壁降解酶活性进行了连续测定,以研究几丁质酶等在这一寄生互作体系中的可能作用。结果表明:葡聚糖酶活性变化表现活跃,且随寄生过程呈增加趋势,配对法T检验结果表明,第10d的处理与对照酶活性差异达到最大;几丁质酶、蛋白酶活性变化表现较低,而纤维素酶未检测得到。酶学动态变化与之前石蜡切片显微观察的结果在时间上表现一致;认为葡聚糖酶可能是粉红黏帚霉67-1菌株寄生核盘菌菌核的关键酶。 |
| 关 键 词： | 粉红黏帚霉 核盘菌 菌核 重寄生 细胞壁降解酶 |
| 文章编号： | 1672-3538（2007）02-0107-03 |
| 修稿时间： | 2007-03-09 |
Dynamic Studies of Some Cell Wall Degrading Enzymes Involved in Mycoparasitism of Gliocladium roseum 67-1 on Sclerotia of Sclerotinia sclerotiorum |
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| LI Jing-jing,ZHANG Yong-hua,CHEN Qiao-yun,LI Shi-dong,XIE Xiang-ming.Dynamic Studies of Some Cell Wall Degrading Enzymes Involved in Mycoparasitism of Gliocladium roseum 67-1 on Sclerotia of Sclerotinia sclerotiorum[J].Journal of Fungal Research,2007,5(2):107-109. | |
| Authors: | LI Jing-jing ZHANG Yong-hua CHEN Qiao-yun LI Shi-dong XIE Xiang-ming |
| Institution: | 1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; 2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| Abstract: | Gliocladium spp.,a kind of fungi broadly habited in soil,was one important bio-control factor of plant diseases.Previous studies indicated that G.roseum 67-1 parasited and destroyed many plant pathogenic fungi.Here reported are some results of the study of the key enzymes involved in the mycoparasitism of G.roseum 67-1 on Sclerotinia sclerotiorum.Dynamic analysis of the activities of cell wall degrading enzymes showed that the activity of glucanase increased evidently companied with the mycoparasitism process and might play a great role.Meanwhile,the activities of chitinase and proteinase behaved lower and the cellulose activity even could not be detected. |
| Keywords: | Gliocladium roseum Sclerotinia sclerotiorum sclerotia mycoparasitism cell wall degrading enzyme |
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