Fatty acid synthetase assay employing bicyclic diones as substrates. |
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| Authors: | A H Ullman J W Harding H B White |
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| Affiliation: | Department of Chemistry, University of Delaware, Newark, Delaware 19711 USA |
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| Abstract: | A rapid and convenient spectrophotometric assay for fatty acid synthetase is presented. It is based on the NADPH-dependent reduction of compounds such as cis-decalin-1,4-dione by the β-ketoacyl-ACP reductase component of the fatty acid synthetase complex. The assay has applications where rapid, semiquantitative data are desired. |
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| Keywords: | Recipient of Research Career Development Award AM 00152. Address reprint requests to this author. |
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