Characterization and selection of <Emphasis Type="Italic">Lactobacillus</Emphasis> strains for their effect on bile tolerance,taurocholate deconjugation and cholesterol removal |
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Authors: | Min Zhang Xiaomin Hang Xiaobing Fan Daotang Li Hong Yang |
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Institution: | (1) School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R. China;(2) Institute of Bio-medicine, Shanghai Jiao Da Onlly Company Limited, Shanghai, 200233, P.R. China |
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Abstract: | Fourteen Lactobacillus strains of six species were investigated with their characteristics of bile salt tolerance, deconjugation of sodium taurocholate
and cholesterol removal in the spent broth. Meanwhile, a co-precipitation curve of cholesterol with cholic acid at concentrations
ranged 0.0–6.0 μM/ml was involved in the evaluation of cholesterol removal. Results demonstrated that both co-precipitation
and assimilation effects contributed to cholesterol removal during the incubation of these Lactobacillus strains. It was also indicated that the supplementation of bile salts influenced the cholesterol removal, not only as an
essential factor related to co-precipitation but also a critical condition for cholesterol assimilation. Out of all strains
tested, four L. plantarum strains LS12, LS31, Lp501 and Lp529 exhibited a high ability of cholesterol assimilation (maximum 20.76 μg/ml), deconjugation
of sodium taurocholate (maximum 5.00 μM/ml) and bile tolerance. They could be further studied and used as potential probiotics
strains to reduce serum cholesterol in humans |
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Keywords: | Bile tolerance Cholesterol removal Deconjugation of bile salt Lactobacillus |
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