| 梨形环棱螺凝集素的初步研究 |
| |
| 引用本文： | 张吉强,李清漪. 梨形环棱螺凝集素的初步研究[J]. 生物化学与生物物理进展, 1995, 22(3): 281-283 |
| |
| 作者姓名： | 张吉强 李清漪 |
| |
| 作者单位： | 第三军医大学组织胚胎学教研室;西南师范大学生物系 |
| |
| 摘 要： | 通过Sepharose 4B-甲状腺球蛋白亲和层析,从梨形环棱螺Bellamya purificata体内分离到的一种凝集素,不连续PAGE显示其为单一的蛋白质谱带.它能凝集兔、猪、鸭等动物的红细胞,但不能凝集人的A、B、O及AB型血的红细胞和固定后的兔红细胞.其凝集活力可被1.0mol/L的乳糖、半乳糖和60g/L的甲状腺球蛋白抑制,但不能被碱性硼酸缓冲液抑制.对温度变化敏感,有较宽的最适pH范围.
|
| 关 键 词： | 梨形环棱螺 凝集素 理化性质 纯化 |
| 收稿时间： | 1994-04-25 |
| 修稿时间： | 1994-07-16 |
A Preliminary Study on Bellamy purificata Lectin |
| |
| Zhang Jiqiang and Li Qingyi. A Preliminary Study on Bellamy purificata Lectin[J]. Progress In Biochemistry and Biophysics, 1995, 22(3): 281-283 |
| |
| Authors: | Zhang Jiqiang and Li Qingyi |
| |
| Affiliation: | Third Military Medical College;Third Military Medical College |
| |
| Abstract: | By Sepharose 4B-thyroglobulin affinity column chromatography a lectin was purified from Bellamya purificata. It showed a single band on alkaline PAGE. This lectin (BPL) agglutinated red blood cells of rabbit,duck and pig,but not of human blood types or formalin and/or glutaraldehyde-fixed rabbit erythrocytes. Its agglutination activity could be inhibited by 1.0mol/L lactose or galactose as well as 60g/L thyroglobulin, but not by alkaline borate buffer.BPL was temperature-sensitive,while showed a broad optical pH range (pH4.56-9.96). |
| |
| Keywords: | Bellamya purificata lectin physicochemical characterization purification |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
|
点击此处可从《生物化学与生物物理进展》下载全文 |
