Electrophysiological and pharmacological properties of single spinal neurons isolated from adult bullfrogs |
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| Authors: | S Adachi J Oka H Fukuda |
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| Institution: | Department of Toxicology and Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan. |
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| Abstract: | 1. We developed an isolated spinal cell preparation from adult bullfrogs. 2. The average resting membrane potential was -60 mV, and an action potential was activated by positive current injection. 3. The cells retained their tetrodotoxin-sensitive Na+ channels and at least two kinetically different types of K+ channel. 4. Under K(+)-free conditions, responses to GABA were blocked by bicuculline while responses to glycine, taurine or beta-alanine were blocked by strychnine. 5. The potency of excitatory amino acids decreased in the order: kainic acid greater than glutamate greater than NMDA. 6. These studies demonstrated that the isolated cells are applicable for electrophysiological and pharmacological investigations. |
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