Aggregated chondrocytes as a model system to study cartilage metabolism |
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Authors: | G. P. J. van Kampen J. P. Veldhuijzen |
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Affiliation: | Department of Oral Cell Biology, School of Dentistry, Vrije Universiteit, 1007 MC Amsterdam, The Netherlands |
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Abstract: | Differentiated chondrocytes, isolated from chick embryo cartilage, were cultured in monolayer, as aggregate or pellet. Aggregation of chondrocytes was accomplished by incubating 2 × 105 cells in a 5-μl drop of culture medium. Under all three conditions, the cells remained healthy and proliferated during culture. However, matrix production, as indicated by incorporation of [35S]sulphate into glycosaminoglycans, was greater in aggregated chondrocytes than in monolayers or pellets. In addition, aggregates consisting of a well defined number of cells, could easily be manipulated for experiments. Therefore aggregates provide a favourable model system to study factors modulating the metabolism of chondrocytes. |
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