Colonisation of soft lining materials by micro‐organisms |
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Authors: | Sabrina Pavan Paulo Henrique Dos Santos João Neudenir Arioli Filho Denise Madalena Palomare Spolidorio |
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Institution: | 1. Department of Dental Materials and Prosthodontics, S?o Paulo State University, Araraquara School of Dentistry, Unesp, Brazil;2. Department of Dental Materials and Prosthodontics, S?o Paulo State University, Aracatuba School of Dentistry, Unesp, Brazil;3. Department of Physiology and Pathology, S?o Paulo State University, Araraquara Dental School, Araraquara, S?o Paulo, Brazil |
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Abstract: | doi:10.1111/j.1741‐2358.2009.00300.x Colonisation of soft lining materials by micro‐organisms Objective: This study evaluated the in vitro adherence of pathogenic micro‐organisms, Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa, to soft lining materials and their inhibitory effect on these micro‐organisms. Materials and Methods: To measure adherence, specimens of Molloplast B and Ufi Gel P were inoculated 107 colony‐forming units per millimetre (cfu/ml)] with TSB media containing the micro‐organisms. To determine the number of micro‐organisms in the 10?2–10?5 dilutions, 25 μl of the suspension were transferred to plates of selective media. Colony counts of each specimen were quantified (cfu/ml). The surface roughness was measured with a perfilometer to assess the relationship between the adherence of micro‐organisms and surface roughness of each material. For the inhibition test, specimens of materials were placed in agar plates inoculated individually with the micro‐organisms. After 48 h, the inhibition zones around the specimens were measured. Results: None of the materials exhibited inhibition zones. The number of cfu/ml of S. aureus and P. aeruginosa were significantly greater than C. albicans for both materials. The Ufi Gel P exhibited greater adherence of C. albicans than Molloplast B. No correlation was observed between the adherence of micro‐organisms and surface roughness. Conclusion: The surface roughness of the materials is not the only factor governing micro‐organism adherence. |
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Keywords: | soft lining materials micro‐organisms surface roughness colonisation |
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