Differential regulation of CaMKII inhibitor β protein expression after exposure to a novel context and during contextual fear memory formation |
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Authors: | K Radwańska A A Tudor‐Jones K Mizuno G S Pereira W Lucchesi I Alfano A Łach L Kaczmarek S Knapp K Peter Giese |
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Institution: | 1. Centre for the Cellular Basis of Behaviour, Institute of Psychiatry, King's College London, London, UK;2. Nencki Institute of Experimental Biology, Warsaw, Poland;3. Neuroscience Centre, Department of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil;4. The Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK |
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Abstract: | Understanding of the molecular basis of long‐term fear memory (fear LTM) formation provides targets in the treatment of emotional disorders. Ca2+/calmodulin‐dependent protein kinase II (CaMKII) is one of the key synaptic molecules involved in fear LTM formation. There are two endogenous inhibitor proteins of CaMKII, CaMKII Nα and Nβ, which can regulate CaMKII activity in vitro. However, the physiological role of these endogenous inhibitors is not known. Here, we have investigated whether CaMKII Nβ protein expression is regulated after contextual fear conditioning or exposure to a novel context. Using a novel CaMKII Nβ‐specific antibody, CaMKII Nβ expression was analysed in the naïve mouse brain as well as in the amygdala and hippocampus after conditioning and context exposure. We show that in naïve mouse forebrain CaMKII Nβ protein is expressed at its highest levels in olfactory bulb, prefrontal and piriform cortices, amygdala and thalamus. The protein is expressed both in dendrites and cell bodies. CaMKII Nβ expression is rapidly and transiently up‐regulated in the hippocampus after context exposure. In the amygdala, its expression is regulated only by contextual fear conditioning and not by exposure to a novel context. In conclusion, we show that CaMKII Nβ expression is differentially regulated by novelty and contextual fear conditioning, providing further insight into molecular basis of fear LTM. |
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Keywords: | α CaMKII amygdala CaMKII Nβ context fear memory hippocampus |
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