Feeding response of Aedes aegypti and Anopheles dirus (Diptera: Culicidae) using out‐of‐date human blood in a membrane feeding apparatus |
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| Authors: | Jinrapa Pothikasikorn Rapee Boonplueang Chalermchai Suebsaeng Rungpetch Khaengraeng Theeraphap Chareonviriyaphap |
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| Affiliation: | 1. Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand;2. Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand;3. Blood Bank, Department of Pathology, Faculty of Medicine Ramathibodi Hospital Mahidol University, Bangkok 10400, Thailand;4. Environmental Science Programme, Department of Applied Science, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok, Thailand |
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| Abstract: | The colonization of Aedes aegypti and Anopheles dirus was performed using out‐of‐date human blood from a blood bank as a nutritional supply dispensed from a common artificial feeder. Preserved human blood was collected and used for feeding on days 5, 15, and 25 after date of expiration and dispensed from a common artificial feeder to rear the mosquitoes. Ae. aegypti had a feeding rate of 78.7, 62, and 18% at the respective intervals while An. dirus had a rate of 80, 56.8, and 7.3% on the same respective days. Direct feeding on live hamsters resulted in a rate of 96 and 90% for Ae. aegypti and An. dirus, respectively. Although egg production rates decreased from the day 5 feeding to the day 25 feeding, all of the developmental stages resulting from An. dirus fed at day 5 and 15 showed insignificant differences when compared with direct feeding on the blood of a hamster. |
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| Keywords: | Aedes aegypti Anopheles dirus colonization out‐of‐date blood artificial feeding Thailand |
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