Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium |
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| Authors: | Solari Claudia Losino Noelia Luzzani Carlos Waisman Ariel Bluguermann Carolina Questa Maria Sevlever Gustavo Miriuka Santiago Barañao Lino Guberman Alejandra |
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| Affiliation: | aDepartment of Endocrinology and Metabolism, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan;bAging Regulation, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan;cDepartment of Obstetrics and Gynecology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan;dDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan;eDepartment of Gastroenterology and Hepatology, Saiseikai Suita Hospital, Osaka, Japan;fDepartment of Medical Biochemistry, Kobe Pharmaceutical University, Kobe, Japan;gInstitute of Bio-Response Informatics, Kyoto, Japan |
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| Abstract: | Senescence marker protein-30 (SMP30) plays an important role in intracellular Ca2+ homeostasis. The aim of the present study was to investigate the effects of estrogens on liver apoptotic damage and changes in SMP30 expression induced by a high saturated fatty acid diet (HSFD). Ovariectomized mice (OVX) and sham-operated mice (SHAM) were randomly divided into five groups: SHAM fed a normal diet (SHAM/ND), SHAM fed HSFD (SHAM/HSFD), OVX fed ND (OVX/ND), OVX fed HSFD (OVX/HSFD) and OVX fed HSFD with 17β-estradiol (E2) supplementation using an implanted slow-release pellet (OVX/HSFD + E2). After 8 weeks, markers of endoplasmic reticulum (ER) stress and apoptosis, and levels of tumor necrosis factor-α (TNFα and SMP30 expression were investigated. Compared with SHAM/ND, OVX/HSFD mice showed significantly increased spliced X-box protein-1 (s-XBP1), phosphorylated eukaryotic initiation factor-2α (p-eIF2α), glucose-regulated protein 78 (GPR78), C/EBP homologous protein (CHOP), cytosolic cytochrome c, caspase-3 activity, and TNFα, and significantly decreased SMP30. These differences in OVX/HSFD mice were restored to the levels of SHAM/ND mice by E2 supplementation. These results suggest that E2 supplementation attenuates HSFD-induced liver apoptotic death in ovariectomized mice by up-regulating SMP30. |
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| Keywords: | Senescence marker protein-30 17β-Estradiol Endoplasmic reticulum stress Apoptosis Saturated fatty acids Nonalcoholic steatohepatitis |
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