| 具有群体感应抑制活性的海洋细菌的筛选 |
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| 引用本文： | 丁碧婷,陆叶倩,董昆明,王小治,周晓见,靳翠丽,缪莉.具有群体感应抑制活性的海洋细菌的筛选[J].微生物学通报,2012,39(1):0002-0008. |
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| 作者姓名： | 丁碧婷 陆叶倩 董昆明 王小治 周晓见 靳翠丽 缪莉 |
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| 作者单位： | 1. 扬州大学 环境科学与工程学院 江苏 扬州 225127
2. 扬州大学 环境科学与工程学院 江苏 扬州 225127;中国科学院上海药物研究所新药研究国家重点实验室 上海 201203 |
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| 基金项目： | 国家自然科学基金项目(No. 41076097, 41006097, 41106113); 新药研究国家重点实验室开放基金项目(No. SIMM1106KF-09); 江苏省高校自然科学基金项目(No. 10KJB170013, 11KJB170011) |
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| 摘 要： | 【目的】从海洋环境中筛选出能有效抑制细菌群体感应的活性菌株,为以致病菌群体感应为靶点的新型疗法提供新的天然产物资源。【方法】以紫色杆菌(Chromobacteriumviolaceum)为报告菌,采用滤纸片法和双层软琼脂法相结合的筛选模型进行群体感应抑制活性菌的筛选。【结果】通过对美国圣璜岛海域海绵中分离出来的272株海洋细菌群体感应抑制活性的筛选,得到了具有抑制紫色杆菌素产生的细菌51株,其中74号菌株抑制效果最好,具有进一步研究的价值。【结论】海洋细菌中有很多具有抑制细菌群体感应效应的菌株,是天然群体感应抑制剂的潜在来源。
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| 关 键 词： | 群体感应抑制活性 紫色杆菌 海洋细菌 |
Screen for the quorum sensing inhibitory activity from marine bacterial isolates |
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| DING Bi-Ting,LU Ye-Qian,DONG Kun-Ming,WANG Xiao-Zhi,ZHOU Xiao-Jian,JIN Cui-Li and MIAO Li.Screen for the quorum sensing inhibitory activity from marine bacterial isolates[J].Microbiology,2012,39(1):0002-0008. |
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| Authors: | DING Bi-Ting LU Ye-Qian DONG Kun-Ming WANG Xiao-Zhi ZHOU Xiao-Jian JIN Cui-Li and MIAO Li |
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| Institution: | 1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China;1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China;1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China;1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China;1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China;1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China;1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China; 2. Key Laboratory of Drug Research, Shanghai Institute of Material Medical, Chinese Academy of Sciences, Shanghai 201203, China |
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| Abstract: | Objective] Quorum sensing inhibitory activity of some marine bacterial isolates was investigated to provide potential novel origins of natural products for the promising anti-bacterial therapeutic approaches targeting on the quorum sensing system of some pathogens. Methods] In this study, we used Chromobacterium violaceum as the report-strain to screen for the quorum sensing inhibitory activity from some marine bacterial isolates by disc diffu-sion assay and double-layer soft agar assay. Results] In total, 272 bacteria strains isolated from the sponge tissues collected around the San Juan Island had been tested. The results showed that 51 isolates exhibited the quorum sensing inhibitory activity. Among the active strains, bacterium No.74 had the strongest activity, which is deserved the further study on the isolation and identification of quorum sensing inhibitors. Conclusion] Many marine bacterial isolates exhibit quorum sensing inhibitory, which indicated that marine bacteria are also a po-tential source for the natural quorum sensing inhibitors. |
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| Keywords: | Quorum sensing inhibitory activity Chromobacterium violaceum Marine bacteria |
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