致病菌烟曲霉新基因Afu4g13170生孢致毒相关性初步研究

【目的】对烟曲霉Afu4g13170基因功能进行初步研究。【方法】利用Double-jointPCR方法和一步基因敲除技术,构建Afu4g13170基因缺失突变株。【结果】序列比对表明烟曲霉Afu4g13170蛋白与构巢曲霉Ani04163蛋白和新型隐球菌Gib2蛋白的氨基酸序列相似性为88.6%;表型分析表明基因破坏使突变株生长迟缓、梗基伸长、孢子分化能力下降,产孢推迟、产孢量减少,色素产生量降低;色谱分析显示基因缺失突变株的产毒能力下降。【结论】烟曲霉Afu4g13170基因可以作为控制曲霉致毒的一个靶位点。

The function of the Afu4g13170 gene in conidiation and gliotoxin production of pathogenic Aspergillus fumigatus

Objective] The function of the uncharacterized gene Afu4g13170 in Aspergillus fumigatus was ascertained.Methods] Double-joint PCR method and one-step gene deletion technique were used to generate the A.fumigatus ΔAfu4g13170 mutant.Results] Amino acid sequence alignment shows that the predicated Afu4g13170 protein has a similarity of 88.6% to Gib2 protein in Cryptococcus neoformans and Ani04163 protein in A.nidulans.The pheno-typic analysis indicated that deletion of Afu4g13170 gene results in growth defect and incom-plete development of phialides with elongated metulae,delayed and reduced sporulation,and less pigment production.Chromatographic analysis suggested that gliotoxin production is blocked partly by knocking out the Afu4g13170 gene.Conclusion] Afu4g13170 gene may be used as one of target sites to control the virulence of A.fumigatus.