Role of polyamines in the cytokinin-dependent physiological processes II. Modulation of polyamine levels during cytokinin-stimulated expansion of cucumber cotyledons |
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Authors: | Jolanta Legocka Anna ?arnowska |
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Institution: | (1) Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, al. Niepodległości 14, 61-713 Poznań |
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Abstract: | The cucumber cotyledon expansion test was used as a model system to study a possible relationship between cytokinin and polyamines.
When kinetin was applied to excised cotyledons incubated in the dark it caused a marked increase in the activity of arginine
decarboxylase. As a result of ADC action, putrescine content also rose markedly, whereas the level of spermidine and spermine
decreased. However, inhibition of putrescine biosynthesis with D-arginine did not affect cytokinin promotion growth. Applied
alone, putrescine had no significant effect on growth. These results indicate that the large increase in putrescine content
that derives from cytokinin treatment cotyledons is not essential for cytokinin-induced expansion of cotyledons. Addition
of K+ and Ca2+ ions to the cotyledons incubated with cytokinin caused a marked reduction in the putrescine level and ADC activity.
The higher level of putrescine (35 %) and spermine (62 %) bound to chromatin and the large increase (174 %) in spermidine
content bound to ribosomes which derive from cytokinintreated cotyledons in relation to literature data can indicate that
these polyamines may play an important role in gene expression during cytokinin-stimulated expansion of cucumber cotyledons.
The inhibition of cytokinin effect, viz. enlargement of the cotyledons by inhibitors of spermidine biosynthesis, additionally suggessted a possible involvement of
polyamines in cytokinin action. |
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Keywords: | chromatin cotyledon expansion cucumber expansion kinetin polyamines ribosomes |
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