Differentiation of creatine kinase MB and IgA-linked BB isoenzymes on electrophoresis |
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Authors: | A Chen S S Wong |
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Affiliation: | 1. Department of Pathology, Tri-Serive General Hospital, National Defense Medical Center, Taiwan, Republic of China;2. The Biochemistry Program, Department of Chemistry, University of Lowell, Lowell, MA 01854, USA |
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Abstract: | IgA-linked creatine kinase (CK, EC 2.7.3.2) is a macro CK type 1 isoenzyme that has an identical electrophoretic mobility to CK-MB. Its presence has the potential of causing misdiagnosis of myocardial infarction. Mixing anti-CK-B antiserum with the sample prior to electrophoresis did not unequivocally distinguish between the two isoenzymes. Similarly, anti-human IgG and IgM antibodies were also ineffective. However, the IgA-linked isoenzyme band was removed by anti-human IgA antiserum. While anti-CK-M antibodies did not affect the electrophoretic mobility of IgA-linked CK-BB, the antibody eliminated both the CK-MB and CK-MM bands. Thus, specific anti-IgA and anti-CK-M antibodies may be used to establish the presence of the myocardial isoenzyme. |
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