Novel RNA recognition motif domain in the cytoplasmic polyadenylation element binding protein 3 |
| |
| Authors: | Takashi Nagata Mari Takahashi Takanori Kigawa Naohiro Kobayashi Peter Güntert Mikako Shirouzu Shigeyuki Yokoyama Yutaka Muto |
| |
| Institution: | 1. RIKEN Systems and Structural Biology Center, , Tsurumi‐ku, Yokohama, 230‐0045 Japan;2. Structural Energy Bioscience Research Section, Institute of Advanced Energy, Kyoto University, Gokasho, , Uji, Kyoto, 611‐0011 Japan;3. Graduate School of Energy Science, Kyoto University, Gokasho, , Uji, Kyoto, 611‐0011 Japan;4. Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, , Tsurumi‐ku, Yokohama, 230‐0045 Japan;5. Laboratory of Molecular Biophysics, Institute for Protein Research, Osaka University, , Suita, Osaka, 565‐0871 Japan;6. Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, , Yokohama, 230‐0045 Japan;7. Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, , Frankfurt am Main, Germany;8. Frankfurt Institute of Advanced Studies, , Frankfurt am Main, Germany;9. Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, , Hachioji, Japan;10. RIKEN Structural Biology Laboratory, , Tsurumi‐ku, Yokohama, 230‐0045 Japan;11. Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, , Tokyo, 202‐8585 Japan |
| |
| Abstract: | The family of cytoplasmic polyadenylation element binding proteins CPEB1, CPEB2, CPEB3, and CPEB4 binds to the 3′‐untranslated region (3′‐UTR) of mRNA, and plays significant roles in mRNA metabolism and translation regulation. They have a common domain organization, involving two consecutive RNA recognition motif (RRM) domains followed by a zinc finger domain in the C‐terminal region. We solved the solution structure of the first RRM domain (RRM1) of human CPEB3, which revealed that CPEB3 RRM1 exhibits structural features distinct from those of the canonical RRM domain. Our structural data provide important information about the RNA binding ability of CPEB3 RRM1. Proteins 2014; 82:2879–2886. © 2014 Wiley Periodicals, Inc. |
| |
| Keywords: | 3′ ‐UTR CPEB3 CPE GluR2 Mrna RRM NMR structure |
|
|
