Human papilloma virus E6/E7 genes can expand the lifespan of human corneal fibroblasts |
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Authors: | Donna M Peters Nathan Dowd Curtis Brandt Teresa Compton |
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Institution: | (1) Present address: Department of Laboratory Medicine and Pathology, 505 SMI, University of Wisconsin, 1300 University Avenue, 53706 Madison, Wisconsin;(2) Department of Ophthalmology and Visual Sciences, University of Wisconsin, 1300 University Avenue, 53706 Madison, Wisconsin;(3) Present address: Department of Medical Microbiology and Immunology, University of Wisconsin, 1300 University Avenue, 53706 Madison, Wisconsin |
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Abstract: | Summary Human corneal fibroblasts were infected with a retroviral delivery vector containing the E6 and E7 genes from human Papilloma
virus type 16 in order to produce cell lines that have an expanded lifespan in culture. Morphologically, some of the trasfected
corneal fibroblast lines appeared to have the normal spindle-shape morphology of diploid fibroblasts, whereas other lines
appeared to have a more elongated morphology. All the cell lines were anchorage-dependent. Cells that had a normal morphology
grew at a rate similar to normal diploid human corneal fibroblasts and had a population doubling time of 48 h. All E6/E7 expressing
cell lines, regardless of morphology, produce types I, III, and V collagen, at levels similar to those observed in the parent
corneal diploid fibroblast. These corneal fibroblast lines will be a usefulin vitro system to study collagen expression and fibril formation, as well as normal stroma development. These results also demonstrate
that the use of E6/E7 genes to expand a cell’s lifespan can be a powerful tool because it does not appear to alter either
the growth rate of the cell or collagen expression. |
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Keywords: | fibroblasts collagen human papilloma virus cell culture corneal stroma |
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