An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton |
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Authors: | Yingfan?Cai Hong?Zhang Yu?Zeng Jianchuan?Mo Jinku?Bao Chen?Miao Jie?Bai Fang?Yan Email author" target="_blank">Fang?ChenEmail author |
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Institution: | (1) College of Bioinformation, Chongqing University of Post and Telecom, 400065 Chongqing, People’s Republic of China;(2) College of Life Science, Sichuan University, 610064 Chengdu, People’s Republic of China |
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Abstract: | A comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through
an optimized high-performance liquid chromatography (HPLC) on a C18 column (4.6 mm x 250 mm, 5 Μm particle) with methanol-0.5%
acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and UV detection at 254 nm. The
method was shown to be highly reproducible, with precision as relative standard deviation (RSD)] and accuracy as relative
mean error (RME)] < 10%, both intra-day and inter-day. Absolute recoveries were > 94%. The results revealed major differences
among the different gland varieties or species of cotton, including the special and ordinary glandless and glandedGossypium hirsutum, G. barbadense, and displayed the precious resources of different glands of extraordinary cotton |
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Keywords: | Cotton (Gossypium) gossypol high-performance liquid chromatography (HPLC) pigment gland |
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