Genetic and Chemical Diversity of High Mucilaginous Plants of <Emphasis Type="Italic">Sida</Emphasis> Complex by ISSR Markers and Chemical Fingerprinting |
| |
Authors: | Sanjog T Thul Ankit K Srivastava Subhash C Singh Karuna Shanker |
| |
Institution: | (1) Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CIMAP-CSIR), Lucknow, 226016, UP, India;(2) Taxonomy and Pharmacognosy Department, Central Institute of Medicinal and Aromatic Plants (CIMAP-CSIR), Lucknow, 226016, UP, India;(3) Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants (CIMAP-CSIR), Lucknow, 226016, UP, India |
| |
Abstract: | A method was developed based on multiple approaches wherein DNA and chemical analysis was carried out toward differentiation
of important species of Sida complex that is being used for commercial preparation. Isolated DNA samples were successfully performed through PCR amplification
using ISSR markers and degree of genetic diversity among the different species of Sida is compared with that of chemical diversity. For genetic fingerprint investigation, selected 10 ISSR primers generating reproducible
banding patterns were used. Among the total of 63 amplicons, 62 were recorded as polymorphic, genetic similarity index deduced
from ISSR profiles ranged from 12 to 51%. Based on similarity index, S. acuta and S. rhombifolia found to be most similar (51%). High number of species-specific bands played pivotal role to delineate species at genetic
level. Investigation based on HPTLC fingerprints analysis revealed 23 bands representing to characteristic chemicals and similarity
index ranged from 73 to 91%. Prominent distinguishable bands were observed only in S. acuta, while S. cordifolia and S. rhombifolia shared most bands making them difficult to identify on chemical fingerprint basis. This report summarizes the genotypic and
chemotypic diversity and the use of profiles for authentication of species of Sida complex. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|