Exploring low-cost carbon sources for microbial lipids production by fed-batch cultivation of <Emphasis Type="Italic">Cryptococcus albidus</Emphasis> |
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Authors: | Qiang Fei Ho Nam Chang Longan Shang Jin-dal-rae Choi |
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Institution: | 1.Biochemical Engineering Lab, Department of Chemical and Biomolecule Engineering,KAIST,Daejeon,Korea;2.Eews Biotech,KAIST,Daejeon,Korea;3.College of Biological and Chemical Engineering, Ningbo Institute of Technology,Zhejiang University,Ningbo,China |
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Abstract: | Volatile fatty acids (VFAs), acetic acid, acetates, and ethanol were used as carbon sources for the production of microbial
lipids using Cryptococcus albidus in batch cultures. C. albidus utilized organic acids less than glucose in the production of lipids, resulting in a lipid yield coefficient on VFAs of 0.125
g/g. In a two-stage batch culture, the lipid content increased to 43.8% (w/w) when VFAs were used as the sole carbon source
in the second stage, which was two times higher than that of the batch culture. Furthermore, a 192 h, two-stage fed-batch
cultivation of C. albidus produced a dry cell weight, lipid concentration, and lipid content of 26.4 g/L, 14.5 g/L, and 55.1% (w/w), respectively.
The fed-batch culture model used in this study featured pure VFA solutions, with intermittent feeding, under oxygen-enriched
air supply conditions. This study investigated several alternative carbon sources to reduce the cost of microbial lipids production
and proved the feasibility of using VFAs as the carbon source for the provision of a high lipid content and productivity. |
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