Establishment of adventitious root culture of <Emphasis Type="Italic">Stevia rebaudiana</Emphasis> Bertoni in a roller bottle system |
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Authors: | Rafael Valim Reis Ana Paula Patrão Luis Borges Talita Perez Cantuaria Chierrito Eliezer Rodrigues de Souto Lauro Mera de Souza Marcello Iacomini Arildo José Braz de Oliveira Regina Aparecida Correia Gonçalves |
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Institution: | 1.Departamento de Farmácia,Universidade Estadual de Maringá,Maringá,Brazil;2.Departamento de Agronomia,Universidade Estadual de Maringá,Maringá,Brazil;3.Departamento de Bioquímica e Biologia Molecular,Universidade Federal do Paraná,Curitiba,Brazil |
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Abstract: | Cultures of adventitious roots of Stevia rebaudiana (Bert.) Bertoni were performed in a roller bottle system for the production of both primary and secondary metabolites. Adventitious
roots were induced from 1-cm-long root tip explants derived from in vitro regenerated plantlets on solid Murashige and Skoog
(MS 1962) media supplemented with 10.7 μM of α-naphthaleneacetic acid. These cultures were successfully maintained in the
same medium for 6 months with regular subcultures after 4 weeks. Thereafter, the roots were cut into 1.0- to 1.5-cm-long segments
and transferred to the roller bottle system containing a fresh root tissue culture on liquid MS medium supplemented with 10.7 μM
NAA. The apparatus consisted of a flask rolling system adjusted to 4g, and 3° of flask inclination. The roots were allowed to grow in the absence of light for adaptation and adventitious root
formation. The best conditions for cultivation were investigated, considering culture volume (25 ml), culture period (4 weeks),
salt concentrations in the nutrient medium (33%) and optimal initial inoculum (0.2 g) of S. rebaudiana roots. These results could give important information on how to improve the development of adventitious roots of S. rebaudiana for the production of primary and secondary metabolites. |
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