Twelve polymorphic expressed sequence tags‐derived markers for Plasmopara halstedii,the causal agent of sunflower downy mildew |
| |
Authors: | X GIRESSE D TOURVIEILLE DE LABROUHE S RICHARD‐CERVERA F DELMOTTE |
| |
Abstract: | Twelve expressed sequence tags‐derived markers were isolated from Plasmopara halstedii (Oomycetes), the causal agent of sunflower downy mildew. A total of 25 single nucleotide polymorphisms and five indels were detected by single‐strand conformation polymorphism analysis and developed for high‐throughput genotyping of 32 isolates. There was a high level of genetic diversity (HE = 0.484). Observed heterozygosity ranged from 0 to 0.143 indicating that P. halstedii is probably a selfing species. These markers were also useful in detecting significant genetic variations among French populations (FST = 0.193) and between French and Russian populations (FST = 0.23). Cross‐amplification tests on three closely related species indicated that no loci amplified in other Oomycete species. |
| |
Keywords: | Oomycetes pathotype evolution physiological race SNP SSCP |
|