Detection and validation of EST‐derived SNPs for poplar leaf rust Melampsora medusae f. sp. deltoidae |
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| Authors: | NICOLAS FEAU,MARIE‐JOS E BERGERON,DAVID L. JOLY,FABIEN ROUSSEL,RICHARD C. HAMELIN |
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| Affiliation: | NICOLAS FEAU,MARIE‐JOSÉE BERGERON,DAVID L. JOLY,FABIEN ROUSSEL,RICHARD C. HAMELIN |
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| Abstract: | We report the discovery, characterization and validation of 118 single nucleotide polymorphisms (SNPs) for poplar leaf rust Melampsora medusae f. sp. deltoidae identified using a gene‐targeted approach in an expressed sequence tag (EST) library. We developed a genotyping assay using the iPLEX? primer extension method for two multiplex assays of 28 and 22 SNPs. |
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| Keywords: | candidate genes EST Melampsora rusts molecular adaptation selection SNP |
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