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A novel multiplex PCR method for detecting the major clonal complexes of MRSA in nasal isolates from a Pennsylvania hospital
Authors:Schwalm Nathan D  Verghese Bindhu  Knabel Stephen J
Institution:
  • Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USA
  • Abstract:A novel multiplex PCR was developed which targeted virulence genes associated with the major clonal complexes (CCs) of healthcare- and community-associated methicillin-resistant Staphylococcus aureus (MRSA) in the USA. Most isolates (40/66) were identified as CC 5, while remaining isolates represented CCs 1, 8, 30, 45, 59, 133, and five isolates were not S. aureus.
    Keywords:Clonal complex  MRSA  Multiplex PCR  Nasal swabs
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