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A new methodology for rapid detection of Lactobacillus delbrueckii subsp. bulgaricus based on multiplex PCR
Authors:Nikolaou Anastasios  Saxami Georgia  Kourkoutas Yiannis  Galanis Alex
Affiliation:
  • Applied Microbiology and Molecular Biotechnology Research Group, Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, GR 68100, Greece
  • Abstract:In this study we present a novel multiplex PCR assay for rapid and efficient detection of Lactobacillus delbrueckii subsp. bulgaricus. The accuracy of our method was confirmed by the successful identification of L. delbrueckii subsp. bulgaricus in commercial yoghurts and food supplements and it may be readily applied to the food industry.
    Keywords:Lactobacillus delbrueckii subsp. bulgaricus   Multiplex PCR   tuf gene   Yogurt
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