Chlorimuron ethyl as a new selectable marker for disrupting genes in the insect-pathogenic fungus Metarhizium robertsii |
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| Authors: | Lin Liangcai Wang Fengqing Wei Dongzhi |
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| Affiliation: | State key laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China |
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| Abstract: | A lack of selectable markers was a hindrance in investigating gene function in Metarhizium robertsii. A reliable Agrobacterium-mediated transformation system based on the use of chlorimuron ethyl as the selectable marker was developed which could serve as a useful tool to inactivate genes involved in insect pathogenicity. |
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| Keywords: | Metarhizium robertsii Gene disruption Selectable marker Green fluorescent protein |
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